| Name | Human Tumor Brain Tumor Lysate |
|---|---|
| Supplier | ABBIOTEC |
| Catalog | 450227 |
| Category | Tissue Lysate |
| Prices | $175.00 |
| Sizes | 100 µg |
| Applications | WB |
| Species | Human |
| Tissue | Brain |
| Disease | Tumor |
| Description | Brain tumor lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol. |
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